The separation of algae extracts in a Thin Layered Chromatography used Matsjeh method, covering plate preparation, sample application, plate development and visualization. In addition, the pigment types of brown algae, Sargassum Polycystum (C. Agardh), were β-caroten, pheofitine, chlorophyll-a and lutein. The pigment types of extracts dissolved difference were β-caroten, xanthofil (zeaxanthin and lutein), chlorophyll-a and chlorophyll-c. The antibacterial activity test used Kirby-Bauer method. Results indicated that the extracts of S. Polycystum, had antibacterial activity on four test bacteria, Escherichia coli, Klebsiella pneumonia, Salmonella paratiphy b and Staphylococcus aureus.
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